Analysis of Cryptococcal Extracellular Vesicles: Experimental Approaches for Studying Their Diversity Among Multiple Isolates, Kinetics of Production, Methods of Separation, and Detection in Cultures of Titan Cells
Dual Fuel Rangecooker ABSTRACT Extracellular vesicles (EVs) produced by members of the Cryptococcus genus are associated with fundamental processes of fungal physiology and virulence.However, several questions about the properties of cryptococcal EVs remain unanswered, mostly because of technical limitations.We recently described a fast and efficie