ANALYSIS OF CRYPTOCOCCAL EXTRACELLULAR VESICLES: EXPERIMENTAL APPROACHES FOR STUDYING THEIR DIVERSITY AMONG MULTIPLE ISOLATES, KINETICS OF PRODUCTION, METHODS OF SEPARATION, AND DETECTION IN CULTURES OF TITAN CELLS

Analysis of Cryptococcal Extracellular Vesicles: Experimental Approaches for Studying Their Diversity Among Multiple Isolates, Kinetics of Production, Methods of Separation, and Detection in Cultures of Titan Cells

Dual Fuel Rangecooker ABSTRACT Extracellular vesicles (EVs) produced by members of the Cryptococcus genus are associated with fundamental processes of fungal physiology and virulence.However, several questions about the properties of cryptococcal EVs remain unanswered, mostly because of technical limitations.We recently described a fast and efficie

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Trichostatin A stabilizes the expression of pluripotent genes in human mesenchymal stem cells during ex vivo expansion.

BACKGROUND: Mesenchymal stem cells (MSCs) have been Coats considered as ideal cells for the treatment of a variety of diseases.However, aging and spontaneous differentiation of MSCs during culture expansion dampen their effectiveness.Previous studies suggest that ex vivo aging of MSCs is largely caused by epigenetic changes particularly a decline o

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